Journal: International journal of biological macromolecules

Article Title: Disulfide bond and crosslinking analyses reveal inter-domain interactions that contribute to the rigidity of placental malaria VAR2CSA structure and formation of CSA binding channel.

doi: 10.1016/j.ijbiomac.2022.11.258

Figure Lengend Snippet: Fig. 8. The binding capacity of full-length VAR2CSA ectodomain is lower than the ectodomain lacking NTS. The binding of NTS-DBL6ε and DBL1x-DBL6ε to CSPG was measured in triplicates by ELISA. The wells of 96-well microtiter plates were coated with CSPG (200 ng/ml) and blocked with BSA and then incubated with 1:2 serially diluted solutions of NTS-DBL6ε or DBL1x-DBL6ε. The levels of bound proteins were measured using anti-cMyc antibody and HRP- conjugated secondary antibody. Data are a representative of three independent experiments using different batches of purified proteins. KD values were determined by plotting OD vs protein concentration using GraphPad Prism v9.4.1. KD ± SE: 41.5 ± 3.9 (DBBL1x-DBL6ε); 59.9 ± 6.8 (NTS-DBL6ε).

Article Snippet: Unbound proteins in the coated wells were removed by washing with 100 μl of PBST and the plates were incubated with 1:1000 diluted mouse anti-cMyc monoclonal antibody (Cat No. NB600-302, NOVUS Biologicals, Centennial, CO), and washed three times with 100 μl PBST.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Purification, Protein Concentration

Journal: Cell

Article Title: Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

doi: 10.1016/j.cell.2019.12.024

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: goat anti-c-Myc Antibody , Novus Biochemicals , Cat# NB600-338 RRID:AB_10001879.

Techniques: Virus, Purification, cDNA Synthesis, Recombinant, Plasmid Preparation, Luminex, Labeling, Software

Journal: Cell and Tissue Research

Article Title: Allergic airway inflammation induces upregulation of the expression of IL-23R by macrophages and not in CD3 + T cells and CD11c + F4/80 − dendritic cells of the lung

doi: 10.1007/s00441-021-03538-0

Figure Lengend Snippet: List of antibodies

Article Snippet: Goat polyclonal anti-c-Myc , Novus Biologicals, USA , 1:200.

Techniques: